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Oxidized glutathione (GSSG) is produced upon reduction of hydroperoxide by GPX, and is recycled to its reduced state by GR and NADPH.The oxidation of NADPH to NADP+ is accompanied by a decrease in absorbance at 340 nm

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(B) NIR fluorescence images of HepG2, HepG2 with inhibitor, and LO2 cell lines incubated with probe -Fuc-DCM (10 M) at 37C for 2 h, imaging wavelength range from 605 to 725 nm, ex = 560 nm