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Use a hard and fast approach when pushing the needle (bevel and shaft) into the skin, going in at a 90 degree angle (see video below)

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It also modulates the activity of metalloproteinases (enzymes that break down extracellular matrix proteins) and their inhibitors, creating a balanced environment where damaged tissue is cleared while new tissue is being built

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Standard allometric scaling (BSA factor ~12.3) would suggest far lower human doses