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One gram of cecal contents of each mouse was dissolved in 3 ml sterilized solution A (containing 20 % glycerol and 1.8 % sodium chloride) which was diluted in polyPeptone Yeast Extract Medium (PY culture medium) and then cultured at 37 C and 200 rpm for 12 h to allow anaerobic bacteria to proliferate

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Rev Endocr Metab Disord (2014) 15(3):1817.10.1007/s11154-014-9289-5 63 VellaACamilleriMRizzaRA