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Surface expression was measured on the Attune NxT Flow Cytometer, data were analyzed using FlowJo 10.8.1 and expressed as % positivity and MFI

Purity of cagrilintide was determined using the following reversed phase high performance liquid chromatography (RP-HPLC) where the samples were analysed using a Kinetex C18, 2.6 pm column (4.6 x 150 mm) with a gradient elution of eluent A consisting of 90% v/v 0.09 M phosphate solution, pH 3.6 and 10% v/v acetonitrile, and eluent B consisting of 60% v/v acetonitrile and 20% v/v isopropanol

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Apr 2012;6(2):172-187

Safety and tolerability The safety evaluation was conducted using data from the safety analysis set, comprising 12 subjects