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Absolute quantitation of intracellular metabolite concentrations by an isotope ratio-based approach

To further validate our transcriptomic findings at the protein level, we assessed expression of key enzymes involved in glutathione biosynthesis, including GCLC, GCLM, and GLRX ( Figures 6E - G )

Compound identification was based on RT and at least one single reaction monitoring (SRM) transition that matched the standards used to build the calibration curve

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This may be primary, due to a genetic transport problem, or secondary, related to another illness, malnutrition, kidney dialysis, or certain medications