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It is important to understand that these in vitro models express a different hormonal cocktail and respond to different chemical stimuli than intestinal L-cells in vivo (53, 54)

The inhibition, which shows dose dependency, is exerted via interactions with receptors in the brain that are accessible via leaks in the blood-brain barrier of the circumventricular organs, mainly the postrema area, the subfornical organ and the median eminence [10, 11]