(4) status of inflammation and immunological markers, neurotrophic and growth factors, and (5) concentrations of proteins related with the pathogenesis of PD or other compounds
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CTK solution (0.25% trypsin + 1 mg/mL collagenase IV + 1 mM CaCl 2 + 20% KSR in DPBS ()) was prepared with trypsin ((2.5%), no phenol red, Thermo Fisher Scientific Inc.), collagenase (type IV, powder, Thermo Fisher Scientific Inc.), calcium chloride dihydrate (Fujifilm Wako Pure Chemical Corporation), Knock Out TM Serum Replacement (KSR, Thermo Fisher Scientific Inc.)
doi: 10.3390/cancers12010153 66
Every batch of Melanotan-II 10mg is independently tested by a third-party analytical laboratory using HPLC for purity and mass spectrometry for identity confirmation