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Although significant progress has been made in the pretreatment of samples and the instrumentation, which has allowed the development of more sensitive, precise, and accurate analytical methods, there are still some aspects that need improvement, such as detection and quantification of peptides with low concentrations in complex matrices and identification and characterization of biomarkers, especially for peptides with short half-lives, to obtain reference materials, especially those synthesized at home, in order to create strategies for the rapid detection of peptides, which are continually entering the doping market, among others