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All real-time PCRs were performed three times and all changes in gene expression were reported as multiples of increases relative to the control while GAPDH was used as an internal control and relative gene expression between the experimental groups were determined using MxPro software

It is also important to consider if the teenager in question has finished growing, as isotretinoin has the potential to slow bone growth in teenagers who have not finished growing

This guide is educational, not medical advice

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If the increase from 0.5mg to 1.0mg triggers significant nausea, stepping to 0.75mg first can ease the transition