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BA is most likely a clinical phenotype resulting from a number of prenatal or perinatal insults to the hepatobiliary tree, and this disease is underpinned by a number of key pathobiological factors (KPFs) either singly or more often in combinations

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After 72 h treatments, 0.5 g ml 1 propidium iodide (Sigma-Aldrich, P4864) and 1.4 g ml 1 Hoechst 33342 (Invitrogen, H3570) were added to cells