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Population Doubling Level Calculation To calculate the PDL, a formula was used as follows: n = 3.32 (log UCY log I ) + X , where n is the final PDL at the end of a given subculture, UCY is the cell yield at that point, I is the cell number used as inoculum to begin that subculture, and X is the PDL of the inoculum used to initiate the subculture being quantitated ( SA--Galactosidase Staining Cells were seeded in 6-well microplates at 4 10 4 cells/well

Experimental studies in animals provide additional insight: GLP-1 analogs increased testosterone synthesis and improved sperm mitochondrial integrity in diabetic and obese models

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8 This effect increased with duration of therapy, and patients had an unclear prophylactic supplementation response

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