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The ability of the antioxidants in a sample to block or diminish 2,2-azinobis (3-ethylbenzothiazoline-6-sulfonic acid, or ABTS) and the blue-green ABTS radical (ABTS+) that is formed is the broad basis for the total antioxidant capacity (TAC) test, which is measured at 505 nm

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and significantly upregulated mRNA expression of IL-6 and IL-17c in intestinal tissue (Arimatsu et al., 2014)

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Shaking can shear the peptide bonds and damage the molecules