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To examine whether sulfonylation of the cysteine residues affects the enzyme activity of OsDHAR, we grew Escherichia coli cells harbouring single genes [ OsDHAR (WT), OsDHAR (C20A) and ScSRX ] and dual genes [ OsDHAR (WT) + ScSRX and OsDHAR (C20A) + ScSRX ]

The intensities of the bands were quantified via Image Lab, version 5.2.1 (Bio-Rad Laboratories)

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Bone loss caused by iron overload in a murine model: importance of oxidative stress