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The sections were then washed with PBS and treated with secondary antibody (diluted in PBS containing 0.3% Triton X-100) for 2 h at room temperature

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Because IGF-1 is normally found at low levels in MetS and T2D, maybe because of insulin cessation to inhibit IGFBP-1 production by the liver and because of decreased liver IGF-1 secretion by insulin stimulation, as insulin resistance prevails in the liver