Cell lines Hepa1-6 (mouse hepatocarcinoma cell line) and HepG2 (human hepatocarcinoma cell line) were purchased from KAC and cultured in DMEM (Wako) containing 10% FBS (Biowest)
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As shown in Figure 7 , UMAP clustering ( Figure 7A ) clearly identifies multiple cellular populations in healthy kidneysincluding endothelial cells (EC), fibroblasts (FIB), vascular smooth muscle cells/pericytes (VSM/P), immune cells (IMM), podocytes (POD), and proximal tubule cells (PT)establishing the cellular expression context of ferroptosis-related genes
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Therapeutic advantages and challenges of targeting central GLP-1 The potential therapeutic benefits of targeting the brains GLP-1 system are significant
