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After acclimation, the following metrics are recorded for 3 min: total distance traveled, time spent in the central zone, and the number of center entries
The metabolic disorders are mainly manifested as an energy imbalance and abnormal glucose and lipid metabolism
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For determination of the midpoint potentials E of roGFP variants OxD roGFP or, alternatively, the degree of reduction ReD roGFP = 1 OxD roGFP was plotted against the calculated redox potentials of the respective lipoic acid redox buffers and all data points were fitted to a sigmoidal dose-response curve using GraphPadPrism5 (GraphPad Software, www.graphpad.com)
