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A statistically significant decrease was observed only in Sequence 2 (i.e., GSH-C4-first), from baseline to the intermediate visit (p = 0.002)

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Moreover, increased ROS and NO production and proinflammatory pathways (TNF, IL-6, and IFNG) may damage the epithelial and endothelial TJs (53)

This problem refers especially to environmental samples and could be solved by proper sample preparation, such as application of sample filtration (glass filers, syringe filters) and extraction (Ramirez et al