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PKA phosphorylates hormone-sensitive lipase (HSL), promoting the hydrolysis of triglycerides into free fatty acids (FFA) and glycerol

In a notable phase II clinical trial published in The Lancet (2008), Tesofensine demonstrated dose-dependent weight loss of up to 10.6% over 24 weeks in obese individualssubstantially higher than placebo or many existing weight-loss drugs

However, increasing structural and compositional complexity introduces challenges related to long-term safety evaluation, regulatory approval, and reproducible manufacturing, highlighting the need for rigorous validation frameworks [183]

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