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DNA fragmentation assay Terminal deoxynucleotidyl transferase-mediated dUTP nick-end labeling (TUNEL) was used to detect DNA fragmentation in oocytes after maturation and in expanded blastocysts

Safety Concerns: Excretion in Breast Milk and Infant Exposure Tirzepatide is a larger peptide molecule that may have limited ability to cross into breast milk compared to smaller pharmaceutical compounds, yet manufacturers have not published definitive data quantifying this transfer rate

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Furthermore, this work offers robust scientific evidence that can inform improved strategies for the prevention and treatment of tissue injury