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Patients who enter my clinic share their past experiences with yo-yo dieting, where a person starts and stops unsustainable diets, leading to cycles of weight loss and gain, along with the impact it can have on their body and self-esteem

Upon reaching confluence, cultures were incubated with the adipogenesis induction cocktail (growth medium supplemented with 5 g ml 1 insulin, 1 M dexamethasone, 0.5 mM isobutylmethyxanthine and 1 M rosiglitazone) for day 12 of differentiation