The two mutation-grafted redesigned proteases were expressed to a much greater level (more than 24-fold) than either E(4130)A2 or WT BoNT/E resulting in 4.5-fold and 3.9-fold higher product formation levels for D2 + (4130)A2 and D3 + (4130)A2, respectively (Fig
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In the absence of sponsor-validated stability data, the following general principles apply to reconstituted injectable peptides in UK aseptic practice (as reflected in NHS Specialist Pharmacy Service guidance on aseptically prepared products): Reconstituted solutions prepared outside a licensed aseptic unit carry a higher risk of microbial contamination and chemical degradation Beyond-use dates must be assigned based on validated data, not on analogy with other products If no validated beyond-use date is available, the product should be used immediately or discarded It is important to note that antimicrobial preservatives in a diluent do not guarantee chemical stability of the peptide itself
