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Cluster analysis (Figure S5B) demonstrated clear separation between groups: controls clustered with high Bacteroidota/Actinomycetota representation, ASD models formed a distinct Firmicutes-dominated cluster, and hCiPSCs-treated animals occupied an intermediate position

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The quenching effect of urea is found to be the most superior (39%) in comparison to glucose, ascorbic acid, dopamine and cholic acid which have fluorescence responses of about 5, 12, 16 and 29%, respectively [46, 55,56,57,58,59,60]