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This process was primarily driven by the abnormal proliferation of Fusobacteriota and Bacteroidota: Bacteroidota could efficiently degrade host mucins and polysaccharides, providing essential carbon and energy sources for the community, while Fusobacteriota might exploit their secreted virulence factors, such as leukotoxins and extracellular proteases, to disrupt the mucosal structure 117

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Then, with a different syringe, he drew up about a quarter of a milliliter of the solution and injected it into his lower abdomen

Afterwards, ApoE-DBCO, in an equimolar amount to N 3 -PEG 3.4k -PDHD, was introduced into the solution and allowed to incubate for an additional 4 h to facilitate conjugation