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Therefore, novel approaches are required to overcome resistance and enhance sensitivity

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Further research revealed that T - 5224 reduced the phosphorylation of components in the PI3K and AKT signaling pathways, suggesting that T - 5224 may induce ferroptosis in MM cells by regulating the PI3K/AKT signaling pathway (Tang S

Then, the cells were washed and suspended in Roswell Park Memorial Institute-1640 medium (RPMI-1640, Sigma-Aldrich) supplemented with 2 g/L sodium bicarbonate, 10% fetal bovine serum (FBS, Sigma-Aldrich), 2% glutamine, and 100 U/mL penicillin-0.1 mg/mL streptomycin (Sigma-Aldrich) and incubated in vitro with RPMI medium (control), tert -Butyl hydroperoxide (tBHP: 200 M) or tBHP plus Ab (22.5 mg/mL) for 30 min at 37C

As our bodies age, they begin to produce less GSH, which is a potential precursor of greater damage by free radicals and stress