The only problem is a rather poor reproducibility in the quantitative evaluation of MPO as a biomarker across laboratories
Adipocyte size analysis was performed using the Keyence BZ-X Analyzer software based on brightfield images of H&E-stained paraffin sections
In brief, 6,250 cells were seeded into 96-well white cell culture-treated plates (Falcon, 353296), treated for 24 h as indicated and labelled following the manufacturers instructions
Jackson MJPS, Bolaos J, Bruckdorfer R, Carlssen H, Elliot RM, Flier J, Griffiths HR, Heales S, Holst B, Lorusso M, Lund E, Moskaug JO, Moser U, Di Paola M, Polidori MC, Signorile A, Stahl W, Via-Ribes J, Astley SB: Antioxidants, reactive oxygen and nitrogen species, gene induction and mitochondrial function
While delayed gastric emptying is most prominent in the initial period of GLP-1 RA therapy, studies showing normalization of gastric emptying remain uncertain (Ushakumari and Sladen 2024)