Further, the SOE PCR products were cloned into FastDigest BshTI/FastDigest BglII (Thermo Fisher) linearized pEGFP-C2 vector using ClonExpress II One Step Cloning Kit (C112, Vazyme) to obtain the six sorting vectors, pEGFP-GST-GPI DAF , pEGFP-GST-GPI BY55 , pEGFP-GST-GPI CEAM7 , pEGFP-GST-TMD ITB3 , pEGFP-GST-TMD ITA5, and pEGFP-GST-TMD ITAV
2024 Sep 4:ard-2024-225565
Glutathionylation of C23 (peptide VVVFIKPT C PYCR), and C8 (peptide MAQEFYN C K) was readily detectable following incubation with 1 mM GSSG, while glutathionylation of C79 and C83 (peptide D C IGG C SDLISMQQTGELMTR) was more prominently detected in response to 50 mM GSSG
[DOI] [PubMed] [Google Scholar] 37.Inchingolo A.M., Malcangi G., Inchingolo A.D., Mancini A., Palmieri G., Di Pede C., Piras F., Inchingolo F., Dipalma G., Patano A
87 LiuH.WangH.ShenviS.HagenT