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Importantly, carnosine was consistently downregulated in both pneumonia models as it is mechanistically linked to histidine and thiamine metabolism (Figures 2F, G), which suggests that carnosine may serve as a critical pneumonia-associated metabolite

Cell lines and cell culture conditions Human GBM cell lines including U87, T98G, LN229, LN18, A172, and luciferase-expressing U87 cells were maintained in Dulbeccos modified Eagles medium (DMEM) supplemented with 10% bovine calf serum (HyClone)

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10.3389/fendo.2021.667066 158