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The female mice (homozygous for Hba tm1(HBA)Tow and heterozygous for Hbb tm2(HBG1,HBB*)Tow and Hbb tm3(HBG1,HBB)Tow [h/h:: A / S ], sickle-cell trait mice) and male mice (homozygous for both Hba tm1(HBA)Tow and Hbb tm2(HBG1,HBB*)Tow [h/h:: S / S ], sickle SCD mice) were bred to generate SCD mice

Subcutaneous injection into abdominal fat or near the site of injury (as commonly practiced by self-experimenters) is the most reported approach, but this is not a clinical protocol

Additionally, specific protein 1 (Sp1) was also considered as a crucial transcription element to facilitate ACSL4 expression by binding to the promoter region of ACSL4 (Li et al

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