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Although one mole of ATP is used by hexokinase and an additional mole of ATP by phosphofructokinase, the net gain is 2 moles of ATP per mole of glucose, because a total of 4 moles of ATP is formed per mole of glucose by phosphoglycerate kinase and pyruvate kinase
Oostenbrink C, Villa A, Mark AE, van Gunsteren WF (2004) A biomolecular force field based on the free enthalpy of hydration and solvation: the GROMOS force-field parameter sets 53A5 and 53A6
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