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SH9H-2024-T246-1)

Growth assay by dilution spotting For growth assay, the different strains were grown overnight in minimal ammonia medium without Uracil/Leucine/histidine and reinoculated in fresh medium to an OD600 of 0.1 without Uracil/Leucine/histidine depending upon the selection marker and grown for 6 hr

CRF antagonists show anxiolytic effects in a variety of animal models (143)

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