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After 12 h incubation, cells were treated with PBS, Ru(II)-OH, NP2, or NP4 for 24 h (Ru 10 M), washed with PBS and incubated with Annexin/PI reagent in the dark for 15 min at 25 C

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DSIP works faster, with sleep pressure reported within hours, but even its full effects on sleep architecture normalization develop over multiple doses

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