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Primer sequences used were as follows: GAPDH (F: CCTCGTCCCGTAGACAAAATG, R: TGAGGTCAATGAAGGGGTCGT);ANP (F: CCGATAGATCTGCCCTCTTGAA, R: GCTGTTGCAGCCTAGTCCACT);BNP (F: GGAGGCGAGACAAGGGAGAA, R: CCAGCGGTGACAGATAAAGGAA);-MHC (F: ATGAGGAGTAGCTCTTGTGCTACC, R: CCACCTAAAGGGCTGTTGC);All primers were synthesized by RiboBio (Guangzhou, China)

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