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Prostate (2009) 69(6):66288

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Notably, AHCY is the only reversible enzyme in the methionine cycle and as such is a key regulator of the SAM to SAH molar ratio, commonly referred to as the methylation index or indicator of methylation capacity. [67] When SAH and homocysteine concentrations increase faster than methionine regeneration, SAM utilizing enzymes are inhibited by SAH and the SAM concentrations then act in a feed-forward mechanism to allosterically activate the B6-dependent cystathionine beta-synthase (CBS) which metabolizes homocysteine through the transsulfuration pathway and allows the methionine cycle to continue [68,69]

Equal amounts of protein were separated by 10% sodium dodecyl sulfatepolyacrylamide gel electrophoresis and transferred onto polyvinylidene difluoride membranes

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