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Mean pixel intensities at 405 and 488 nm were quantified via Fiji/ImageJ and the ratio 488/405 nm was calculated
However, because cuproptosis is a relatively recently defined cell death modality, the extent to which this mechanism operates across diverse physiological and pathological contexts remains to be fully established
The next day, slides were washed with TBST, followed by staining with the secondary antibodies of goat anti-rat IgG conjugated with TRITC (Jackson Lab) and goat anti-mouse IgG conjugated with FITC
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