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Pain perception is highly individual and influenced by multiple factors including needle phobia, injection technique, anatomical site selection, and previous experience with injectable medications

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Together with the pharmacodynamic data, this PK knowledge may help in selecting dosing regimens needed to achieve target exposures that maximize efficacy and minimize toxicity, advancing the translation of 7,8-DHF therapy from preclinical to clinical studies

Firstly, our strategy does not involve the expression of 3CD protease and therefore eliminates 3CD-caused cellular toxicity and/or incomplete cleavage of P1 by 3CD