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Itaconate is transported to the cytosol where several enzymes of glucose metabolism are inhibited including aldolase A (encoded by the ALDOA gene), glyceraldehyde-3-phosphate dehydrogenase (encoded by the GAPDH gene), lactate dehydrogenase (encoded by the LDHA gene), enolase (encoded by the ENO1 gene), and the PKM2 isoform of pyruvate kinase (encoded by the PKM gene)

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Gai Y, Zhang MY, Ji PY, You RJ, Ge ZJ, Shen W, et al

Without the Drug Affinity Complex (DAC), this peptide has a short half-life of about 30 minutes to 2 hours