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At 2 h after plating, the plating medium was replaced with fresh culture medium consisting of DMEM supplemented with 1 mM sodium pyruvate, 100 units/ml penicillin, 100 g/ml streptomycin, 0.2 % (vol/vol) of bovine serum albumin fraction V (instead of FBS), 100 nM dexamethasone, and 1 nM insulin (SigmaAldrich #I9278)

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The shots have gained popularity after being used by celebrities, such as Rihanna and Katy Perry, because they're promoted as a means to boost energy levels

Mice were euthanized 24 h post-injection, and major organs were harvested for analysis