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doi: 10.1056/NEJMoa050151

Clinical guidance for intramuscular injections points to a 22 to 25 gauge needle, given at a 90-degree angle, with the length chosen so it reaches muscle without going too deep

holding the needle in place ~5 seconds before withdrawal

In the rat model of induced IVD degeneration via artificial annular tears, 60 subsequent nerve sprouting following disc degeneration highly correlated to grip strength impairment was observed, most likely mediated by axial hypersensitivity