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Real-time quantitative polymerase chain reaction Total RNA was extracted from cells using the Trizol reagent (Takara Bio Inc, Japan) according to the manufacturers instructions

Free radicals generated by ultraviolet radiation, pollution, and other environmental stressors cause oxidative damage to cellular structures, including DNA, proteins, and lipids that maintain healthy skin function

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Glutathioneis essential for the immune system's proper functioning and is vital in building and repairing tissue

If bacteria are to survive, then they must obtain iron from their environment