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Seikkula J, Olson ME

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Following standard protocols provided by the manufacturer, the anti-GST antibody (GE) was first immobilized on the chip surface via amine coupling to the free carboxyl groups on the chip surface using standard NHS/EDC procedures with PBS-P+ (20 mM phosphate buffer with 2.7 mM KCl, 137 mM NaCl and 0.05% Surfactant P20, pH 7.4) as the Running Buffer

Consider what happens when tissue is injured: the immediate area often experiences reduced blood flow due to damage to existing vessels

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