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uPAR-28z CAR-T cells were generated, and a single injection effectively eliminated senescent cells and improved nonalcoholic fatty liver disease (NASH)-induced liver fibrosis in young mice

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doi: 10.3390/ijms20081811

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Cells were fixed with formaldehyde and incubated with anti-Myc antibody (Abcam, catalog number ab9106) to detect DARPin expression and -p53 antibody DO-I (Santa Cruz Biotechnology) to detect p53 expression, followed by incubation with the secondary antibodies Alexa Fluor 568 anti-rabbit (Life Technologies) and Alexa Fluor 647 anti-mouse (Life Technologies)