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In vitro studies have demonstrated that GHK-Cu increases the production of metalloproteinases and their inhibitors (TIMPs), enabling coordinated matrix remodeling rather than simply increasing matrix deposition

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Earlier animal studies, observational research, and smaller human trials had suggested that GLP-1 receptor agonists might also influence alcohol-related behaviors, providing the rationale for this study

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This imbalance may have reduced the treatment effect estimate and resulted in the observed heterogeneity