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(2) To remove hydrophobic substances, wash the column with 3-4 bed volumes of 70% ethanol or 2 bed volumes of 1% Triton X-100 followed by 5 bed volumes of PBS (pH 7.4) washed

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This can lead to: Greater risk of hypoglycemia , especially when fasted Long-term insulin resistance if misused Higher chance of receptor desensitization However, it has fewer injection site issues , as its typically administered subcutaneously or intramuscularly away from the training site