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Tissues were then incubated in 10, 20, and 30% sucrose in 1 PBS, embedded in OCT compound (Tissue-Tek, Sakura Finetek, 4583), snap frozen on dry ice, and then stored frozen at 80C

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The diffraction pattern created by the bright auroras, as well as the moon Io (just off to the left, not visible in the image), form a complex background of scattered light around Jupiter

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