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Each sample was brought up to a final volume of 50 L

Statistical analyses Statistical analyses were performed using STATA version 18 (College Station, TX) via the secured VINCI workspace

For oral semaglutide (Rybelsus), the typical starting dose is 3 mg once daily for the first month, increasing to 7 mg once daily after 30 days if tolerated

2 In addition to lifestyle modifications (diet and exercise), many pharmacologic strategies have been proposed to counter iatrogenic weight gain, including appetite suppressants (eg, pro-dopaminergic agents such as phentermine, stimulants, and amantadine), pro-anorectant anticonvulsants (eg, topiramate or zonisamide), opioid receptor antagonists (eg, olanzapine/samidorphan or naltrexone) and oral hypoglycemics such as metformin

The chest tube was then removed, and the rat was allowed to recover from the anesthesia and the endotracheal tube was removed