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All procedures involving animals were conducted humanely and were performed by or under the direction of trained personnel

Copper has a complex role in oxidative biology participating in both prooxidant chemistry (where free or weakly bound copper can catalyze damaging reactive-oxygen-species generation) and antioxidant chemistry (as the cofactor of Cu/Zn superoxide dismutase)

One of the commonly reported microbiome differences between obese/HFD-fed animals and lean controls is an increased Firmicutes : Bacteroidetes ratio 43,44

TNFalpha mechanically sensitizes masseter muscle afferent fibers of male rats

the kidneys and liver are particularly vulnerable, with lead causing kidney dysfunction and mercury leading to liver injury