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Chromatographic separation was performed on a Ultraphere ODS RP-18 analytical column (5 m, 4.6 mm x 15 cm, Beckman), fitted with a C18 (4 3 mm) security guard cartridge system (Phenomenex, USA)
PORCN, located in the endoplasmic reticulum, can add palmitoleate groups to Wnt proteins, and it is a member of the membrane-bound O-acyltransferases (MBOATs)
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Effects of oxidative stress induced by environmental relevant concentrations of fluoxetine on the embryonic development on Danio rerio