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Loss of puf-8 promotes accumulation of both endogenous LET-60 and a GFP:H2B reporter under the control of the let-60 3UTR in mitotic germ cells as well as in early meiotic cells, suggesting direct regulation of let-60 by PUF-8 (Vaid et al., 2013)

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Samples of blood and stimulated whole saliva were collected from fasted participants in the morning hours between 8:00 AM and 10:00 AM for the further analyses