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In the present study, we compared the effects of TDDS and the injectable administration of semaglutide on body weight, fat distribution, metabolic rate, organ function, and behaviors in C57BL/6J male mice

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RT-qPCR analysis revealed that 5 days of glutamine withdrawal strongly upregulated expression of CTH, CBS, and SLC7A11 (Figure 21C)

Then an appropriate amount of protein loading buffer was added and electrophoretic separation was performed on SDS-PAGE gels (7.5% or 15%)