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The infected cells were subsequently resuspended in lysis buffer (containing 50 mM Tris, 10% sucrose, 50 M ATP, 1 mM phenylmethylsulfonyl fluoride (PMSF), 5 mM dithiothreitol (DTT), 1% NP40, 10 mM imidazole, 1 protease inhibitor and phosphatase inhibitor, pH 7.0, with HCl)

doi: 10.1080/19490976.2018.1474322 222 WagnerJMaksimovicJFarriesGSimWHBishopRFCameronDJet al

An update on chronic complications of diabetes mellitus: from molecular mechanisms to therapeutic strategies with a focus on metabolic memory

Therefore, the inhibition of xCT-mediated cystine transport, cystine depletion, limited cysteine biosynthesis, impaired GSH synthesis, or inactivation of GPX4 can induce ferroptosis [49]

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